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jorvis avatar jorvis commented on July 18, 2024

Is it possible for you to post your GFF anywhere? This is usually due to the GFF not following the specification (which also supports our need for a proper validator in biocode, already requested in issue #17)

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ncpalmateer avatar ncpalmateer commented on July 18, 2024

The paths to the input files are:
/local/scratch/ncpalmateer/silva_lab/p67/BV115/BV115.gff3
/local/scratch/ncpalmateer/silva_lab/p67/BV115/BV115.fasta

Also, the gff file was made from GMAP.

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jorvis avatar jorvis commented on July 18, 2024

This is caused by features in your input file which have an mRNA but no associated CDS. I've commited a fix for this in commit 9870938 but it will still write empty-length entries in FASTA (no good) until I also patch the write_fasta_from_gff3.py script. Looking at that now.

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jorvis avatar jorvis commented on July 18, 2024

Example of one of the problematic features: F42B2A9795D365B6F68334D3D403D376.path1

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jorvis avatar jorvis commented on July 18, 2024

As noted in commit ebb1688, the export script now prints a warning and skips any features which have 0-length.

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