Comments (1)
It seems your barcodes are of length 9 and have wildcards (N
s) in them, yet you select seven nucleotides from the read.
Perhaps you can try to remove the three N
s from your barcodes and select six nucelotides from the read (e.g., -s 4 -e 9
).
from demultiplex.
Related Issues (20)
- demultiplex match outputs all input reads to each barcode HOT 3
- dealing with conflicts HOT 5
- Output HOT 12
- tags 5' and 3' in two different files usage HOT 1
- Paired end support for match. HOT 2
- Dual barcodes with match HOT 3
- demultiplexing illumina with forward and reverse tag HOT 1
- Degenerated constructs HOT 5
- Does demultiplex support hamming distance? HOT 1
- All reads go into unknown HOT 4
- Is it possible for the barcode to contain non-ATCG characters? HOT 1
- demultiplex unknown results HOT 4
- pairwise2.py doesn't work. Biopython deprecated it. HOT 4
- Does demultiplex pick up where it left off? HOT 1
- Does this tool works for Cell hashing data generated for cell ranger multi? HOT 3
- Demultiplex cannot separate read-pairs by R1 barcode and R2 barcode simultaneously if at least either of the two is found HOT 10
- Demultiplex v1.2.2 misses barcodes starting with a (series of) DELetions aka supposedly incompletely amplified PCR products HOT 11
- Demultiplex with i7 index on dual index FASTQ HOT 2
- demultiplex does not find any barcodes if -e NUM is larger than lenghth of the shortest actually provided barcode in a TSV file HOT 9
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from demultiplex.