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huangyh09 avatar huangyh09 commented on August 11, 2024

Hi, the briekit-event-filter is only for the skipping exon events. You may need to define your own filters for the A3SS (alternative 3' splice site) event, which we haven't looked into.

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Ivymanman avatar Ivymanman commented on August 11, 2024

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jihongma1010 avatar jihongma1010 commented on August 11, 2024

hi, I got this error when I run
briekit-event -a /disk1/reference_genome/hg38_ucsc_ercc/Gencodev32_hg38_ercc_refseq.gtf -o /disk2/brie_rnasplicing_bc25/bc25_as/
Making GFF alternative events annotation...

  • Input annotation files: /disk1/reference_genome/hg38_ucsc_ercc/Gencodev32_hg38_ercc_refseq.gtf
  • Output dir: /disk2/brie_rnasplicing_bc25/bc25_as/
    ('Reading table', '/disk1/reference_genome/hg38_ucsc_ercc/Gencodev32_hg38_ercc_refseq.gtf')
    Traceback (most recent call last):
    File "/home/JHma/miniconda3/envs/RNASEQ/bin/briekit-event", line 11, in
    load_entry_point('briekit==0.2.2', 'console_scripts', 'briekit-event')()
    File "build/bdist.linux-x86_64/egg/briekit/events/event_maker.py", line 175, in main
    File "build/bdist.linux-x86_64/egg/briekit/events/event_maker.py", line 69, in defineAllSplicing
    File "build/bdist.linux-x86_64/egg/briekit/events/event_maker.py", line 39, in prepareSplicegraph
    File "build/bdist.linux-x86_64/egg/briekit/events/parseTables.py", line 85, in populateSplicegraph
    File "build/bdist.linux-x86_64/egg/briekit/events/parseTables.py", line 64, in readTable_gff
    UnboundLocalError: local variable 'exons' referenced before assignment

Could you help me? Thanks

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