Missing parent: GO:0004583 dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity subclasses about go-ontology HOT 3 CLOSED

Thanks both I will add!

from go-ontology.

Feedback from Ben Kellman (expect contacted by Frederique Lisacek):

I am not an experienced curator, so I can't comment with certainty on the meaning of parentage. However, I have spent time in glycan biosynthesis, so I may be of help there.

Since you work in yeast, you are likely aware of ALG genes. For citations, PMID:11425794 provides some useful citations in the broader context of Dolichol-P-P-GlcNAc2Man9Glc3 biosynthesis. The final paragraph references the yeast knockouts that uncovered the alg genes responsible for the process.

Here is a more modern figure summarizing the process and context:

Essentials of Glycobiology [Internet]. 4th edition. Cold Spring Harbor (NY)

I'm pointing at the whole process because when I think of the dolichol-P-glycan precursor to N-glycosylation, I rarely think specifically of the terminal tri-glucose additions. Over a dozen enzymes are responsible for building N-glycan precursor. That said, these three enzymes are similar in that they share a precursor, glucose.

In summary, I think your proposal makes sense due to the similarity of these reactions. And, provided some structure already exists surrounding the context of these three reactions. E.g.:
N-glycan biosynthesis
N-glycan precursor biosynthesis
dol-P synthesis
cytosolic
glcnac additions
DPAGT1, 13/14
mannose additions
ALG1, 2, 2, 11, 11
in ER
flip dol-PP-glycan into ER
mannose additions
ALG3, ALG9, ALG12, ALG9
glucose additions
ALG6, ALG8, ALG10
dolichol to protein transfer ....

Hope this is helpful,
Ben

from go-ontology.

I agree with the suggestion to create a new superclass for these three genes.

from go-ontology.