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jackhump avatar jackhump commented on July 18, 2024

this is pretty odd. Every time I've run prepare_results.R with the hg19 bed files I get two warnings about inconsistent records but I've never had a seg fault. Have you updated to the latest version of bedtools?

If these weird chromosome names are the case then maybe we could get rid of them when creating the bed files?

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davidaknowles avatar davidaknowles commented on July 18, 2024

That warning may just be a coincidence, not sure. I'll try updating and see if it helps!

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davidaknowles avatar davidaknowles commented on July 18, 2024

Hmm so upgrading bedtools from v2.25.0 to v2.26.0 didn't fix this.

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davidaknowles avatar davidaknowles commented on July 18, 2024

I removed the "weird" chromosome names from gencode_hg19_all_introns.bed.gz. I don't get the warning anymore but I still get the segfault, so I don't think this was actually the root of the problem. @jackhump can you try running on your machine and see if you the same problem?

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davidaknowles avatar davidaknowles commented on July 18, 2024

I tried with a new annotation_code generated with wrangle_annotation.sh and still have the same issue.

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davidaknowles avatar davidaknowles commented on July 18, 2024

So I'm starting to think this is a bug in bedtools, I've raised an issue here: arq5x/bedtools2#576

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davidaknowles avatar davidaknowles commented on July 18, 2024

I've reworked prepare_results.R to use dplyr joins instead of bedtools now so we avoid these issues. I've also changed the CLI to be a bit simpler to document.

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