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reccej avatar reccej commented on August 26, 2024

Hmmm, looks like the link changed to only accept https connections. We'll have to update the script with updated links, thanks for the info

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reccej avatar reccej commented on August 26, 2024

For example: https://www.imgt.org/download/V-QUEST/IMGT_V-QUEST_reference_directory/Homo_sapiens/IG/IGHV.fasta works

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reccej avatar reccej commented on August 26, 2024

@Ch-rode @yaober I've just pushed an update to the repository that fixes all of the links in the setup script (as well as another minor bug).

I haven't gotten all of the changes pushed to pip yet but if you git clone this repository and do the local install method the setup script should work now:
git clone https://github.com/crowelab/PyIR cd PyIR/ pip3 install --user .

Let me know if something is wrong

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Ch-rode avatar Ch-rode commented on August 26, 2024

@reccej thanks a lot, everything is working now.
I am sorry to bother again, I was also wondering if you added the isotypes as well. I cloned the repo but i do not find them in the output.
thanks again

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reccej avatar reccej commented on August 26, 2024

I thought I did, the isotype databases are in the source but maybe they're not getting properly compiled to a BLAST library -- I'll have to confer with @myersml5 about it. I've been mostly out of the sequencing game for 3 years and he's my go-to now

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reccej avatar reccej commented on August 26, 2024

If you wanna get funky with it, look in the pyir/data/crowelab_data folder for the files 'human_gl_C.fasta' and 'human_TCR_C.fasta'. They can be used to build a BLAST database which should be able to be fed into PyIR using the --germlineC flag, but it may be a spotty implementation

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