Comments (2)
jcmcnch
commented on May 27, 2024
1
Hi Lior,
Thank you so much for your detailed reply, and I'm sorry I never replied. This will be a great reference going forward, and I hope using your explanations I can now figure out how to construct the json config. If I'm still running into issues, I'll be sure to reach out.
Jesse
from pheniqs.
moonwatcher
commented on May 27, 2024
Hi @jcmcnch
First, welcome and thank you for using Pheniqs. Now about your config files...
-
You can always validate your JSON to see that it is syntactically correct. I provide a simple wrapper for the python validator with Pheniqs. You can just download json_lint.py and use it to lint your json. That will give you a very clear error if something is wrong, i.e. what line and what is the problems. To use it do something like
original_config.json|./json_lint.py. You should also use some text editor that can provide highlighting and collapsing of JSON documents (like Atom . It will make inspecting the document much easier. -
You don't really need the pheniqs-io-api.py with your config. The purpose of this api is to add the individual output directives to each barcode when you want to split the output into files by barcode. since you already did that, the only thing the api added is the undetermined class, which is implicitly added anyway if you didn't specify it. I linted both files and ran a diff
> },
> "undetermined": {
> "output": [
> "JMSNRSCF_undetermined_s01.fastq.gz",
> "JMSNRSCF_undetermined_s02.fastq.gz",
> "JMSNRSCF_undetermined_s03.fastq.gz",
> "JMSNRSCF_undetermined_s04.fastq.gz"
> ]linting the files sorts the JSON dictionaries which makes comparing two files much easier.
-
You also don't really need
-F fastq --compression gzthis will only provide a default output format for outputs that are not explicitly specified. Since you already added thefastq.gzextensions it is unnecessary. -
if you try and validate your config file you will notice you get an error.
pheniqs mux --config original.json --validate
Configuration error : sample decoder : duplicate barcode sequence AGTCAA
if you search for AGTCAA in your file you will notice it shows up twice. once in @11_15 and once in @2_120. You can't specify the same barcode sequence for two classes since that would be ambiguous. same for ATGAGC, GTGGCC, CGCCTG, CCGTCC. Once we remove those offending classes (again, barcode sequence must be unique per decoder, it is identifying the class), your next problems is
pheniqs mux --config original.json --validate
Configuration error : incorrect number of output URLs in channel
Your config file specifies the output template has 2 tokens 0:: and 3::, basically the complete first and 4th input segments.
"template" : {
"transform" : {
"token" : [ "0::", "3::" ]
}
},but in all your output directives you specify 4 output files. The number of output files in the output directive must be the number of segments you specify in your template.
- Once all those problems have been corrected your config file validated. I will provide the adjusted file and the validation report at the end of this reply but to answer your question about the output please notice the following lines from the validation report:
Barcode undetermined
ID : JMSNRSCF:undetermined
PU : JMSNRSCF:undetermined
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Segment No.0 : /dev/stdout?format=fastq&compression=gz&level=5
Segment No.1 : /dev/stdout?format=fastq&compression=gz&level=5
the output directive in pheniqs config files is cascaded. That means that if you specify it in the root of the document it will provide the default for anything further deeper. Since you explicitly provided the output directive for each barcode that leaves only the undetermined class to inherit the default. You didn't provide an output directive in the document root so it reverted to the global default which is SAM format to stdout. Since you provided the -F fastq --compression gz command line parameters that switched to gzip compressed FASTQ format. What you got over stdout are the unclassified reads. if you look at the folder where you executed this you will probably find the multiple fastq files for the individual barcodes. Notice you can specify /dev/null as output if you want to discard a certain output so you can specify the undetermined to have an output with /dev/null if you are not interested in it.
- One last comment. If you run pheniqs with
--compileit will output your compiled config file. That is a config file where all the implicit runtime parameters have been computed and added. It might be a little big but it allows you to see exactly what pheniqs understood from your instruction and what will be executed. To compile your config file usepheniqs mux --config original.json --compile
If you still have questions, please don't hesitate to ask. We appreciate your feedback and are here to help.
Regards
Lior.
{
"PL": "ILLUMINA",
"PM": "NovaSeq_2x250_UCDavis_run064",
"base input url": ".",
"filter incoming qc fail": true,
"flowcell id": "JMSNRSCF",
"input": [
"JMSNRSCF_S1_L001_R1_001.fastq.gz",
"JMSNRSCF_S1_L001_I1_001.fastq.gz",
"JMSNRSCF_S1_L001_I2_001.fastq.gz",
"JMSNRSCF_S1_L001_R2_001.fastq.gz"
],
"report url": "211123_JMSNRSCF_demux_sample_report.json",
"sample": {
"algorithm": "pamld",
"codec": {
"@11_15": {
"LB": "11_15",
"barcode": [
"AGTCAA"
],
"output": [
"JMSNRSCF_11_15_s01.fastq.gz",
"JMSNRSCF_11_15_s02.fastq.gz"
]
},
"@11_200": {
"LB": "11_200",
"barcode": [
"ATGTCA"
],
"output": [
"JMSNRSCF_11_200_s01.fastq.gz",
"JMSNRSCF_11_200_s02.fastq.gz"
]
},
"@11_25": {
"LB": "11_25",
"barcode": [
"CGTACG"
],
"output": [
"JMSNRSCF_11_25_s01.fastq.gz",
"JMSNRSCF_11_25_s02.fastq.gz"
]
},
"@2_400": {
"LB": "2_400",
"barcode": [
"ATCTCA"
],
"output": [
"JMSNRSCF_2_400_s01.fastq.gz",
"JMSNRSCF_2_400_s02.fastq.gz"
]
},
"@2_45": {
"LB": "2_45",
"barcode": [
"AGGAAT"
],
"output": [
"JMSNRSCF_2_45_s01.fastq.gz",
"JMSNRSCF_2_45_s02.fastq.gz"
]
},
"@7_120": {
"LB": "7_120",
"barcode": [
"ATTCCT"
],
"output": [
"JMSNRSCF_7_120_s01.fastq.gz",
"JMSNRSCF_7_120_s02.fastq.gz"
]
},
"@7_200": {
"LB": "7_200",
"barcode": [
"GTGGCC"
],
"output": [
"JMSNRSCF_7_200_s01.fastq.gz",
"JMSNRSCF_7_200_s02.fastq.gz"
]
},
"@7_25": {
"LB": "7_25",
"barcode": [
"GTGAAA"
],
"output": [
"JMSNRSCF_7_25_s01.fastq.gz",
"JMSNRSCF_7_25_s02.fastq.gz"
]
},
"@8_200": {
"LB": "8_200",
"barcode": [
"ATGAGC"
],
"output": [
"JMSNRSCF_8_200_s01.fastq.gz",
"JMSNRSCF_8_200_s02.fastq.gz"
]
},
"@8_800": {
"LB": "8_800",
"barcode": [
"CCGTCC"
],
"output": [
"JMSNRSCF_8_800_s01.fastq.gz",
"JMSNRSCF_8_800_s02.fastq.gz"
]
},
"@9_200": {
"LB": "9_200",
"barcode": [
"ACTTCC"
],
"output": [
"JMSNRSCF_9_200_s01.fastq.gz",
"JMSNRSCF_9_200_s02.fastq.gz"
]
},
"@BGT_PL01_rev11": {
"LB": "BGT_PL01_rev11",
"barcode": [
"GCGGAC"
],
"output": [
"JMSNRSCF_BGT_PL01_rev11_s01.fastq.gz",
"JMSNRSCF_BGT_PL01_rev11_s02.fastq.gz"
]
},
"@BGT_PL01_rev12": {
"LB": "BGT_PL01_rev12",
"barcode": [
"TTTCAC"
],
"output": [
"JMSNRSCF_BGT_PL01_rev12_s01.fastq.gz",
"JMSNRSCF_BGT_PL01_rev12_s02.fastq.gz"
]
},
"@BGT_PL01_rev13": {
"LB": "BGT_PL01_rev13",
"barcode": [
"CCGGTG"
],
"output": [
"JMSNRSCF_BGT_PL01_rev13_s01.fastq.gz",
"JMSNRSCF_BGT_PL01_rev13_s02.fastq.gz"
]
},
"@BGT_PL01_rev14": {
"LB": "BGT_PL01_rev14",
"barcode": [
"ATCGTG"
],
"output": [
"JMSNRSCF_BGT_PL01_rev14_s01.fastq.gz",
"JMSNRSCF_BGT_PL01_rev14_s02.fastq.gz"
]
},
"@BGT_PL02_rev15": {
"LB": "BGT_PL02_rev15",
"barcode": [
"TGAGTG"
],
"output": [
"JMSNRSCF_BGT_PL02_rev15_s01.fastq.gz",
"JMSNRSCF_BGT_PL02_rev15_s02.fastq.gz"
]
},
"@BGT_PL02_rev16": {
"LB": "BGT_PL02_rev16",
"barcode": [
"CGCCTG"
],
"output": [
"JMSNRSCF_BGT_PL02_rev16_s01.fastq.gz",
"JMSNRSCF_BGT_PL02_rev16_s02.fastq.gz"
]
},
"@CAP_PL01_rev01": {
"LB": "CAP_PL01_rev01",
"barcode": [
"CGTGAT"
],
"output": [
"JMSNRSCF_CAP_PL01_rev01_s01.fastq.gz",
"JMSNRSCF_CAP_PL01_rev01_s02.fastq.gz"
]
},
"@CAP_PL01_rev02": {
"LB": "CAP_PL01_rev02",
"barcode": [
"ACATCG"
],
"output": [
"JMSNRSCF_CAP_PL01_rev02_s01.fastq.gz",
"JMSNRSCF_CAP_PL01_rev02_s02.fastq.gz"
]
},
"@CAP_PL01_rev03": {
"LB": "CAP_PL01_rev03",
"barcode": [
"GCCTAA"
],
"output": [
"JMSNRSCF_CAP_PL01_rev03_s01.fastq.gz",
"JMSNRSCF_CAP_PL01_rev03_s02.fastq.gz"
]
},
"@CAP_PL01_rev04": {
"LB": "CAP_PL01_rev04",
"barcode": [
"TGGTCA"
],
"output": [
"JMSNRSCF_CAP_PL01_rev04_s01.fastq.gz",
"JMSNRSCF_CAP_PL01_rev04_s02.fastq.gz"
]
},
"@Colette_rev21": {
"LB": "Colette_rev21",
"barcode": [
"TTCGTC"
],
"output": [
"JMSNRSCF_Colette_rev21_s01.fastq.gz",
"JMSNRSCF_Colette_rev21_s02.fastq.gz"
]
},
"@Colette_rev22": {
"LB": "Colette_rev22",
"barcode": [
"CCAACT"
],
"output": [
"JMSNRSCF_Colette_rev22_s01.fastq.gz",
"JMSNRSCF_Colette_rev22_s02.fastq.gz"
]
},
"@Colette_rev23": {
"LB": "Colette_rev23",
"barcode": [
"TCAGTT"
],
"output": [
"JMSNRSCF_Colette_rev23_s01.fastq.gz",
"JMSNRSCF_Colette_rev23_s02.fastq.gz"
]
},
"@Colette_rev24": {
"LB": "Colette_rev24",
"barcode": [
"CTGACC"
],
"output": [
"JMSNRSCF_Colette_rev24_s01.fastq.gz",
"JMSNRSCF_Colette_rev24_s02.fastq.gz"
]
},
"@Delaney_rev19": {
"LB": "Delaney_rev19",
"barcode": [
"GGAACT"
],
"output": [
"JMSNRSCF_Delaney_rev19_s01.fastq.gz",
"JMSNRSCF_Delaney_rev19_s02.fastq.gz"
]
},
"@Delaney_rev20": {
"LB": "Delaney_rev20",
"barcode": [
"CGAAAC"
],
"output": [
"JMSNRSCF_Delaney_rev20_s01.fastq.gz",
"JMSNRSCF_Delaney_rev20_s02.fastq.gz"
]
},
"@Rae_rev05": {
"LB": "Rae_rev05",
"barcode": [
"CACTGT"
],
"output": [
"JMSNRSCF_Rae_rev05_s01.fastq.gz",
"JMSNRSCF_Rae_rev05_s02.fastq.gz"
]
},
"@mystery12264": {
"LB": "mystery12264",
"barcode": [
"ACTGAT"
],
"output": [
"JMSNRSCF_mystery12264_s01.fastq.gz",
"JMSNRSCF_mystery12264_s02.fastq.gz"
]
},
"@mystery4895": {
"LB": "mystery4895",
"barcode": [
"GGGGGG"
],
"output": [
"JMSNRSCF_mystery4895_s01.fastq.gz",
"JMSNRSCF_mystery4895_s02.fastq.gz"
]
},
"@mystery8619": {
"LB": "mystery8619",
"barcode": [
"AGTTCC"
],
"output": [
"JMSNRSCF_mystery8619_s01.fastq.gz",
"JMSNRSCF_mystery8619_s02.fastq.gz"
]
}
},
"confidence threshold": 0.95,
"noise": 0.05,
"transform": {
"token": [
"1::6"
]
}
},
"template": {
"transform": {
"token": [
"0::",
"3::"
]
}
}
}rick:jcmcnch lg% pheniqs mux --config original.json -F fastq --compression gz --validate
Environment
Base input URL .
Base output URL .
Platform ILLUMINA
Quality tracking disabled
Filter incoming QC failed reads enabled
Filter outgoing QC failed reads disabled
Input Phred offset 33
Output Phred offset 33
Leading segment index 0
Default output format fastq
Default output compression gz
Default output compression level 5
Feed buffer capacity 2048
Threads 8
Decoding threads 2
HTSLib threads 8
Input
Input segment cardinality 4
Input segment No.0 : ./JMSNRSCF_S1_L001_R1_001.fastq.gz?format=fastq&compression=gz
Input segment No.1 : ./JMSNRSCF_S1_L001_I1_001.fastq.gz?format=fastq&compression=gz
Input segment No.2 : ./JMSNRSCF_S1_L001_I2_001.fastq.gz?format=fastq&compression=gz
Input segment No.3 : ./JMSNRSCF_S1_L001_R2_001.fastq.gz?format=fastq&compression=gz
Input feed No.0
Type : fastq
Compression : gz
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : ./JMSNRSCF_S1_L001_R1_001.fastq.gz?format=fastq&compression=gz
Input feed No.1
Type : fastq
Compression : gz
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : ./JMSNRSCF_S1_L001_I1_001.fastq.gz?format=fastq&compression=gz
Input feed No.2
Type : fastq
Compression : gz
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : ./JMSNRSCF_S1_L001_I2_001.fastq.gz?format=fastq&compression=gz
Input feed No.3
Type : fastq
Compression : gz
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : ./JMSNRSCF_S1_L001_R2_001.fastq.gz?format=fastq&compression=gz
Output transform
Output segment cardinality 2
Token No.0
Length variable
Pattern 0::
Description cycles 0 to end of input segment 0
Token No.1
Length variable
Pattern 3::
Description cycles 0 to end of input segment 3
Assembly instruction
Append token 0 of input segment 0 to output segment 0
Append token 1 of input segment 3 to output segment 1
Sample decoding
Decoding algorithm pamld
Shannon bound 0
Noise 0.05
Confidence threshold 0.95
Segment cardinality 1
Nucleotide cardinality 6
Transform
Token No.0
Length 6
Pattern 1::6
Description cycles 0 to 6 of input segment 1
Assembly instruction
Append token 0 of input segment 1 to output segment 0
Barcode undetermined
ID : JMSNRSCF:undetermined
PU : JMSNRSCF:undetermined
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Segment No.0 : /dev/stdout?format=fastq&compression=gz&level=5
Segment No.1 : /dev/stdout?format=fastq&compression=gz&level=5
Barcode @11_15
ID : JMSNRSCF:AGTCAA
PU : JMSNRSCF:AGTCAA
LB : 11_15
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : AGTCAA
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_11_15_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_11_15_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @11_200
ID : JMSNRSCF:ATGTCA
PU : JMSNRSCF:ATGTCA
LB : 11_200
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : ATGTCA
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_11_200_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_11_200_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @11_25
ID : JMSNRSCF:CGTACG
PU : JMSNRSCF:CGTACG
LB : 11_25
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : CGTACG
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_11_25_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_11_25_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @2_400
ID : JMSNRSCF:ATCTCA
PU : JMSNRSCF:ATCTCA
LB : 2_400
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : ATCTCA
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_2_400_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_2_400_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @2_45
ID : JMSNRSCF:AGGAAT
PU : JMSNRSCF:AGGAAT
LB : 2_45
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : AGGAAT
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_2_45_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_2_45_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @7_120
ID : JMSNRSCF:ATTCCT
PU : JMSNRSCF:ATTCCT
LB : 7_120
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : ATTCCT
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_7_120_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_7_120_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @7_200
ID : JMSNRSCF:GTGGCC
PU : JMSNRSCF:GTGGCC
LB : 7_200
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : GTGGCC
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_7_200_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_7_200_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @7_25
ID : JMSNRSCF:GTGAAA
PU : JMSNRSCF:GTGAAA
LB : 7_25
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : GTGAAA
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_7_25_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_7_25_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @8_200
ID : JMSNRSCF:ATGAGC
PU : JMSNRSCF:ATGAGC
LB : 8_200
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : ATGAGC
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_8_200_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_8_200_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @8_800
ID : JMSNRSCF:CCGTCC
PU : JMSNRSCF:CCGTCC
LB : 8_800
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : CCGTCC
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_8_800_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_8_800_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @9_200
ID : JMSNRSCF:ACTTCC
PU : JMSNRSCF:ACTTCC
LB : 9_200
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : ACTTCC
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_9_200_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_9_200_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @BGT_PL01_rev11
ID : JMSNRSCF:GCGGAC
PU : JMSNRSCF:GCGGAC
LB : BGT_PL01_rev11
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : GCGGAC
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev11_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev11_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @BGT_PL01_rev12
ID : JMSNRSCF:TTTCAC
PU : JMSNRSCF:TTTCAC
LB : BGT_PL01_rev12
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : TTTCAC
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev12_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev12_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @BGT_PL01_rev13
ID : JMSNRSCF:CCGGTG
PU : JMSNRSCF:CCGGTG
LB : BGT_PL01_rev13
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : CCGGTG
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev13_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev13_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @BGT_PL01_rev14
ID : JMSNRSCF:ATCGTG
PU : JMSNRSCF:ATCGTG
LB : BGT_PL01_rev14
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : ATCGTG
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev14_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev14_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @BGT_PL02_rev15
ID : JMSNRSCF:TGAGTG
PU : JMSNRSCF:TGAGTG
LB : BGT_PL02_rev15
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : TGAGTG
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL02_rev15_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL02_rev15_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @BGT_PL02_rev16
ID : JMSNRSCF:CGCCTG
PU : JMSNRSCF:CGCCTG
LB : BGT_PL02_rev16
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : CGCCTG
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL02_rev16_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL02_rev16_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @CAP_PL01_rev01
ID : JMSNRSCF:CGTGAT
PU : JMSNRSCF:CGTGAT
LB : CAP_PL01_rev01
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : CGTGAT
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev01_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev01_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @CAP_PL01_rev02
ID : JMSNRSCF:ACATCG
PU : JMSNRSCF:ACATCG
LB : CAP_PL01_rev02
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : ACATCG
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev02_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev02_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @CAP_PL01_rev03
ID : JMSNRSCF:GCCTAA
PU : JMSNRSCF:GCCTAA
LB : CAP_PL01_rev03
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : GCCTAA
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev03_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev03_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @CAP_PL01_rev04
ID : JMSNRSCF:TGGTCA
PU : JMSNRSCF:TGGTCA
LB : CAP_PL01_rev04
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : TGGTCA
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev04_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev04_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @Colette_rev21
ID : JMSNRSCF:TTCGTC
PU : JMSNRSCF:TTCGTC
LB : Colette_rev21
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : TTCGTC
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev21_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev21_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @Colette_rev22
ID : JMSNRSCF:CCAACT
PU : JMSNRSCF:CCAACT
LB : Colette_rev22
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : CCAACT
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev22_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev22_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @Colette_rev23
ID : JMSNRSCF:TCAGTT
PU : JMSNRSCF:TCAGTT
LB : Colette_rev23
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : TCAGTT
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev23_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev23_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @Colette_rev24
ID : JMSNRSCF:CTGACC
PU : JMSNRSCF:CTGACC
LB : Colette_rev24
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : CTGACC
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev24_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev24_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @Delaney_rev19
ID : JMSNRSCF:GGAACT
PU : JMSNRSCF:GGAACT
LB : Delaney_rev19
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : GGAACT
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Delaney_rev19_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Delaney_rev19_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @Delaney_rev20
ID : JMSNRSCF:CGAAAC
PU : JMSNRSCF:CGAAAC
LB : Delaney_rev20
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : CGAAAC
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Delaney_rev20_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Delaney_rev20_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @Rae_rev05
ID : JMSNRSCF:CACTGT
PU : JMSNRSCF:CACTGT
LB : Rae_rev05
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : CACTGT
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Rae_rev05_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Rae_rev05_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @mystery12264
ID : JMSNRSCF:ACTGAT
PU : JMSNRSCF:ACTGAT
LB : mystery12264
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : ACTGAT
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_mystery12264_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_mystery12264_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @mystery4895
ID : JMSNRSCF:GGGGGG
PU : JMSNRSCF:GGGGGG
LB : mystery4895
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : GGGGGG
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_mystery4895_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_mystery4895_s02.fastq.gz?format=fastq&compression=gz&level=5
Barcode @mystery8619
ID : JMSNRSCF:AGTTCC
PU : JMSNRSCF:AGTTCC
LB : mystery8619
PL : ILLUMINA
PM : NovaSeq_2x250_UCDavis_run064
Concentration : 0.0306451612903226
Barcode : AGTTCC
Segment No.0 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_mystery8619_s01.fastq.gz?format=fastq&compression=gz&level=5
Segment No.1 : /Users/lg/Desktop/jcmcnch/JMSNRSCF_mystery8619_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.62
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev02_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.61
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_11_200_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.60
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL02_rev15_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.59
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_11_25_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.58
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev03_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.57
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_8_200_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.56
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_8_800_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.55
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev01_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.54
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_2_400_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.53
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_mystery4895_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.52
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_2_400_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.51
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_2_45_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.50
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Rae_rev05_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.49
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_11_15_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.48
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_2_45_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.47
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_9_200_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.46
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_11_15_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.41
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_mystery12264_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.43
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_8_200_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.42
Type : fastq
Compression : gz@5
Resolution : 2
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 4096
URL : /dev/stdout?format=fastq&compression=gz&level=5
Output feed No.38
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_7_25_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.36
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev13_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.34
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Delaney_rev19_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.35
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_11_25_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.33
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_9_200_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.31
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_7_120_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.32
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev04_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.30
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev11_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.29
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev11_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.28
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev12_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.27
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev01_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.26
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev21_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.22
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL02_rev15_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.40
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev03_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.19
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL02_rev16_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.18
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_7_200_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.4
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Delaney_rev20_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.20
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_11_200_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.21
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL02_rev16_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.15
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Delaney_rev20_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.17
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev02_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.16
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_7_25_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.12
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev14_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.13
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev22_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.14
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev21_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.11
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev22_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.5
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Delaney_rev19_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.1
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_mystery8619_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.45
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_7_120_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.44
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev13_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.2
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_mystery12264_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.10
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev23_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.6
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev24_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.9
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev23_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.8
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_mystery4895_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.37
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_8_800_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.7
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Colette_rev24_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.24
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_7_200_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.25
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev12_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.23
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_CAP_PL01_rev04_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.3
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_Rae_rev05_s02.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.39
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_BGT_PL01_rev14_s01.fastq.gz?format=fastq&compression=gz&level=5
Output feed No.0
Type : fastq
Compression : gz@5
Resolution : 1
Phred offset : 33
Platform : ILLUMINA
Buffer capacity : 2048
URL : /Users/lg/Desktop/jcmcnch/JMSNRSCF_mystery8619_s02.fastq.gz?format=fastq&compression=gz&level=5
from pheniqs.
Related Issues (20)
- Install failure with pheniqs-tools (ppkg.py) HOT 2
- error while installing pheniqs under centos 6 using ppkg.py HOT 5
- Pheniqs only processes a small fraction of reads HOT 21
- --help bug HOT 2
- Desirable future features
- EOF error HOT 3
- Citing Pheniqs HOT 3
- Trouble replicating basic behavior HOT 3
- Troubleshooting "SequenceError" error HOT 1
- output knitted and corrected barcodes to fastq HOT 7
- demultiplexing based on primer HOT 7
- Last record missing in barcode corrected BAM file HOT 8
- Quickstart Example not working for me HOT 1
- Quadruple indexing, variable index length HOT 6
- IO error HOT 1
- Tutorial info not correct? HOT 1
- demultiplexing by multiple barcode positions HOT 1
- Pheniqs on very large barcode spaces HOT 1
- Incorrect urls in 'Getting Started' HOT 1
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